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مقاله
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Abstract
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Title:
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Genes expression of NOGO-A and its receptors in amniotic fluid treated human RPE cells
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Author(s):
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Bahareh Safdari,Mozhgan Rezaei Kanavi,Iman Salahshourifar,Zahra-Soheila Soheili
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Presentation Type:
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Poster
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Subject:
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Anatomy & Pathology/ Oncology
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Others:
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Presenting Author:
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Name:
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Bahareh Safdari
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Affiliation :(optional)
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Department of biology ,Tehran Science and Research branch,Islamic Azad University,Tehran,Iran.
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E mail:
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baharsafdari8@gmail.com
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Phone:
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02188474490
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Mobile:
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09128047712
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Purpose:
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To investigate gene expression of NOGO-A and its receptors in human amniotic fluid (HAF)-treated human retinal pigment epithelial (hRPE) cells compared to fetal bovine serum (FBS)-treated cells.
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Methods:
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Gene expressions of NOGO-A and its receptors, NgR1 and lingo-1, were investigated and analyzed in 30% HAF- versus FBS-treated hRPE cells by using real time PCR.
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Results:
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Our results showed that NOGO-A, NgR1 and Lingo-1 are normally expressed in hRPE cells. Gene expressions of NOGO-A, NgR1, and Lingo-1 in HAF-treated hRPE cells were, respectively, 112 (P < 0.001), 20 (P < 0.001), and 11 (P = 0.014) times respected to those of FBS-treated cells.
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Conclusion:
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Increased gene expressions of NOGO-A and its receptors support neural differentiation of hRPE cells in the presence of HAF. Our findings provide further evidences on effectiveness of HAF in trans-differentiation of hRPE cells into neural progenitors and retinal neural cells, which may be of great help in cell replacement therapy.
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Attachment:
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23bahareh-safdari IRAVO poster 2018.pptx
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